During the heating process, prepare picro-sirius red stain solution (PSR). The recipe is as following:0.1% direct red 80 plus 0.1% fast green FCF dissolved in saturated aqueous picric …

The present study provides evidence that, as compared with Sirius Red alone or van Gieson staining, the Sirius Red/Fast Green method is the most sensitive, in terms of both qualitative …

Sirius Red binds to all types of collagen, whereas fast green stains non-collagenous proteins. This method has been applied to the measurement of collagen contents in various tissues.

In bright-field microscopy, collagen is red on a pale-yellow background. Picrosirius red can cause appreciable de-staining of the nuclei. Under polarized light, thick collagen type I fibers appear …

Digital image analysis is performed by using software to quantify total collagen and background based on specific threshold settings for the red, green, and blue channels. Background …

To the best of my knowledge, most users of picro-sirius red are doing research that exploits the enhancement by sirius red of the birefringence of collagen fibres, which is largely due to co …

Feb 26, 2025 · In this blog post, we will explore the fundamentals of picrosirius red staining, including its applications, step-by-step protocol, advantages, limitations, and troubleshooting …

This stain can be performed with or without the fast green for the demonstration of collagen. Sirius red is specific for type 1 collagen in tissues, but also some type 4 and complement under …

Sirius Red and Fast Green is a dye combination used to distinguish collagen from its surrounding materials. Sirius Red specifically binds the [Gly-X-Y]n helical structure of fibrillar collagens, …

Aug 24, 2025 · Picrosirius red staining is a widely used technique in histology and pathology for visualizing collagen fibers, particularly types I and III, in tissue sections. This protocol provides …