Normal phase HPLC is a valuable technique for the separation of polar and hydrophilic compounds and for isomer separation. Normal phase HPLC requires a polar stationary phase combined with non or ...

Operating HPLC at higher than ambient temperatures can improve peak shapes and enable faster run times. Preheating the mobile phase is significant in high temperature liquid chromatography as the ...

Hydroxypropyl methyl cellulose (HPMC) stock solution, 2% (wt/vol; ∼86 K nominal molecular weight; 4,000-cP viscosity; Aldrich); prepare 24 h before use as an aqueous 2% (wt/vol) stock solution by ...

This is the second article in this series discussing HPLC oligonucleotide separations. This article will discuss the separation of oligonucleotides and dsDNAs using a wide-pore, polymer based reversed ...

Any analytical method must strike a balance between its intrinsic scientific potential its capacity for robustness and reliability, particularly in regulated industries. In the case of HPLC, this ...

Pharmaceuticals need to be assessed for stability to support the assigned shelf life. Therefore, when analysing stability samples obtained from these studies, analytical methods are required which are ...

The term liquid chromatography (LC) refers to a class of physicochemical methods which are used to separate and quantify compounds in a sample. LC is made up of a liquid mobile phase, which carries ...

High performance liquid chromatography (HPLC) has become an essential technique in pharmaceutical analysis for the separation, identification, and quantification of pharmaceuticals. Due to its high ...